J., Moya-Salazar V., Rojas-Zumaran Supplementary Material for: Eco-Pap: The Ecological Modification of the Papanicolaou Stain for Sustainable Cervical Cancer Diagnosis <b><i>Objectives:</i></b> To validate the ecological modification of the Papanicolaou stain (Eco-Pap) for the diagnosis of cervical cancer. <b><i>Study Design:</i></b> A prospective study was performed at the Mother and Child “San Bartolomé” Hospital, Lima, Peru. Reagent handling strategies were divided into three phases: we used (1) Harris progressive hematoxylin (for nuclear staining), (2) a polychromatic solution (a mix of EA-36/Orange G-6 to suppress the use of several alcohol baths), and (3) direct mounting (with Entellan® solution). The cellular details were analyzed by the staining quality index, an external quality control, and the Bethesda System 2014. <b><i>Results:</i></b> We evaluated 72,901 cervical smears stained with the Eco-Pap. The validation of the Eco-Pap against the conventional Pap stain was optimal (κ = 0.89; 95% CI: 0.87–0.92), showing a sensibility and specificity of 88.3% (95% CI: 85.1–90.0) and 98.7% (95% CI: 98–99.2), respectively. The Eco-Pap dramatically reduced the environmental pollution caused by 72 L of xylene, hydrochloric acid, and ammonia (6 L each) and mercury oxide. <b><i>Conclusion:</i></b> The Eco-Pap is an innovative and efficient staining method reducing the use of toxic reagents with carcinogenic potential during cervical cancer screening by exfoliative cytology. Cervical cancer screening;Cervical cytology;Papanicolaou test;Eco-Pap;Polycyclic aromatic hydrocarbons;Hydrochloric acid 2018-10-23
    https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Eco-Pap_The_Ecological_Modification_of_the_Papanicolaou_Stain_for_Sustainable_Cervical_Cancer_Diagnosis/7240925
10.6084/m9.figshare.7240925.v1