Supplementary Material for: The Association and Clinical Significance of CDKN2A Promoter Methylation in Head and Neck Squamous Cell Carcinoma: a Meta-Analysis Zhou C. Shen Z. Ye D. Li Q. Deng H. Liu H. Li J. 10.6084/m9.figshare.7246235.v1 https://karger.figshare.com/articles/dataset/Supplementary_Material_for_The_Association_and_Clinical_Significance_of_CDKN2A_Promoter_Methylation_in_Head_and_Neck_Squamous_Cell_Carcinoma_a_Meta-Analysis/7246235 <b><i>Background/Aims:</i></b> The association between cyclin-dependent kinase inhibitor 2A (<i>CDKN2A</i>) hypermethylation and head and neck squamous cell carcinoma (HNSCC) risk has been investigated by a number of studies. However, these studies have not demonstrated consistent results. Moreover, the role of <i>CDKN2A</i> methylation in HNSCC carcinogenesis and its clinical significance remain unclear. <b><i>Methods:</i></b> We performed a systematic meta-analysis based on 72 articles (including 3399 HNSCCs, 668 premalignant lesions, and 2393 normal controls) from the PubMed, Google Scholar, Web of Science, Embase, China National Knowledge Infrastructure and Wanfang databases. <b><i>Results:</i></b> Our study showed a significant increase in the frequency of <i>CDKN2A</i> methylation during HNSCC carcinogenesis (HNSCC vs. normal controls, odds ratio (OR) = 6.72, <i>P</i> < 0.01; HNSCC vs. precancerous lesions, OR = 1.89, <i>P</i> < 0.05; precancerous lesions vs. normal controls, OR = 14.70, <i>P</i> < 0.01). Moreover, <i>CDKN2A</i> methylation was significantly associated with gender (OR = 1.34; <i>P</i> < 0.05) and lymph node metastasis (OR = 2.32; <i>P</i> < 0.01). The area under summary receiver operating characteristic curve (AUC) for diagnosis of HNSCC based on all samples and saliva sample subgroup were 0.77 and 0.96, respectively. Additionally, <i>CDKN2A</i> hypermethylation was significantly associated with shorter overall survival (OS) (hazard ratio (HR) = 1.01, <i>P</i> < 0.05) and recurrence-free survival (RFS) (HR = 1.77, <i>P</i> < 0.05). <b><i>Conclusion:</i></b> Our findings indicate <i>CDKN2A</i> methylation is involved in the carcinogenesis, progression, and metastasis of HNSCC. Furthermore, methylated <i>CDKN2A</i> could be a potential diagnostic and prognostic biomarker for HNSCC. 2018-10-24 08:52:47 CDKN2A Methylation Head and neck squamous cell carcinoma Diagnosis Prognosis Carcinogenesis