Supplementary Material for: Molecular Epidemiology of <b><i>ALK</i></b> Rearrangements in Advanced Lung Adenocarcinoma in Latin America
Arrieta O.
Cardona A.F.
Bramuglia G.
Cruz-Rico G.
Corrales L.
Martín C.
Imaz-Olguín V.
Castillo O.
Cuello M.
Rojas-Bilbao É.
Casas G.
Fernández C.
ArénFrontera O.
Denninghoff V.
Recondo G.
Avilés-Salas A.
Mas-Lopez L.-A.
Oblitas G.
Rojas L.
Piottante A.
Jiménez-García E.
Sánchez-Sosa S.
Sáenz-Frias J.
Lupera H.
Ramírez-Tirado L.A.
Vargas C.
Carranza H.
Astudillo H.
Wills L.B.
Pichelbaur E.
Raez L.E.
on behalf of the CLICaP
10.6084/m9.figshare.7380776.v1
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Molecular_Epidemiology_of_b_i_ALK_i_b_Rearrangements_in_Advanced_Lung_Adenocarcinoma_in_Latin_America/7380776
<b><i>Objective:</i></b> Latin American countries are heterogeneous in terms of lung cancer incidence and exposure to potential carcinogens. We evaluated the frequency and clinical characteristics of <i>ALK</i> rearrangements (<i>ALKr</i>) in Latin America. <b><i>Methods:</i></b> A total of 5,130 lung cancer patients from 10 Latin American countries were screened for inclusion. <i>ALKr</i> detection was performed by fluorescence in situ hybridization (FISH), immunohistochemistry (IHC), and real-time reverse transcriptase-polymerase chain reaction (RT-PCR) to assess method variability. Demographic and clinicopathologic characteristics were analyzed. <b><i>Results:</i></b> Among the 5,130 patients screened, 8.4% (<i>n</i> = 433) had nonevaluable FISH tests. Evaluable FISH analyses revealed positive <i>ALKr</i> in 6.8% (320/4,697) of the study population, which included patients from 9 countries. <i>ALKr</i> distribution for each country was: Mexico 7.6% (79/1,034), Colombia 4.1% (10/242), Argentina 6.0% (153/2,534), Costa Rica 9.5% (13/137), Panama 4.4% (5/114), Uruguay 5.4% (2/37), Chile 8.6% (16/185), Venezuela 8.9% (13/146), and Peru 10.8% (29/268). RT-PCR showed high positive (83.6%) and negative (99.7%) predictive values when compared to the gold standard FISH. In contrast, IHC only showed a high negative predictive value (94.6%). <b><i>Conclusions:</i></b> Although there is a clear country and continental variability in terms of <i>ALKr</i> frequency, this difference is not significant and the overall incidence of <i>ALKr</i> in Latin America does not differ from the rest of the world.
2018-11-26 10:48:04
Non-small-cell lung cancer
Rearrangements
ALK
FISH
Latin America