%0 Generic
%A M., Ebbinghaus
%A Z., Jenei-Lanzl
%A G., SegondvonBanchet
%A H., Stangl
%A M., Gajda
%A R.H., Straub
%A H.-G., Schaible
%D 2018
%T Supplementary Material for: A Promising New Approach for the Treatment of Inflammatory Pain: Transfer of Stem Cell-Derived Tyrosine Hydroxylase-Positive Cells
%U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_A_Promising_New_Approach_for_the_Treatment_of_Inflammatory_Pain_Transfer_of_Stem_Cell-Derived_Tyrosine_Hydroxylase-Positive_Cells/7484741
%R 10.6084/m9.figshare.7484741.v1
%2 https://karger.figshare.com/ndownloader/files/13868150
%2 https://karger.figshare.com/ndownloader/files/13868153
%K Antigen-induced arthritis
%K Catecholamines
%K Inflammation
%K Pain-related behavior
%K Macrophages
%K Arginase-1
%K Tyrosine hydroxylase
%K Dorsal root ganglia neurons
%K Interleukin-4
%X Objectives: The appearance of endogenous tyrosine hydroxylase-positive cells (TH+ cells) in collagen-induced arthritis was associated with an anti-inflammatory effect. Here we investigated putative anti-inflammatory and antinociceptive effects of the transfer of induced, bone marrow stem cell-derived TH+ cells (iTH+ cells) on murine antigen-induced arthritis (AIA). Methods: Bone marrow-derived stem cells were differentiated into iTH+ cells. These cells were transferred to mice immunized against methylated bovine serum albumin (mBSA) 2 days before AIA was induced by injection of mBSA into one knee joint. In AIA control mice and iTH+-treated mice the severity of AIA, pain-related behavior, humoral and cellular responses, and the invasion of macrophages into the dorsal root ganglia were assessed. Results: The intravenous transfer of iTH+ cells before AIA induction did not cause a sustained suppression of AIA severity but significantly reduced inflammation-evoked pain-related behavior. The iTH+ cells used for transfer exhibited enormous production of interleukin-4. A major difference between AIA control mice and iTH+-treated AIA mice was a massive invasion of the dorsal root ganglia by iNOS-negative, arginine 1-positive macrophages corresponding to an M2 phenotype. The differences in other cellular and humoral immune parameters such as release of cytokines from stimulated lymphocytes between AIA control mice and iTH+-treated mice were small. Conclusions: The transfer of iTH+ cells may cause a long-lasting reduction of arthritis-induced pain even if it does not ameliorate inflammation. The invasion of M2 macrophages into the dorsal root ganglia is likely to be an important mechanism of antinociception.
%I Karger Publishers