%0 Generic
%A F., Yazılıtaş
%A F.Z., Oztek-Celebi
%A Ö., Erel
%A E.K., Çakıcı
%A M., Alışık
%A M., Bülbül
%D 2019
%T Supplementary Material for: Dynamic Thiol/Disulphide Homeostasis in Children with Nephrotic Syndrome
%U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Dynamic_Thiol_Disulphide_Homeostasis_in_Children_with_Nephrotic_Syndrome/7680026
%R 10.6084/m9.figshare.7680026.v1
%2 https://karger.figshare.com/ndownloader/files/14280584
%K Anti-oxidants
%K Childhood
%K Nephrotic syndrome
%K Oxidative stress
%K Thiol/disulphide homeostasis
%X Background: There is a growing body of evidence showing that there is an association between oxidative damage and kidney injury in patients with nephrotic syndrome (NS). Dynamic thiol/disulphide homeostasis (DTDH) is an important antioxidant system. The aim of this study was to determine if DTDH could be used as a biomarker of antioxidant status in pediatric NS patients using a novel automated method. Methods: The study included 39 patients with NS and 40 healthy controls. The DTDH level was measured using a calorimetric and automated method developed by Erel and Neselioglu. In the NS group, DTDH was measured during first-attack episode, relapse, and remission. Results: The native thiol, total thiol, and disulphide levels were significantly lower, the native thiol/total thiol ratio was significantly lower, and the disulphide/native thiol and disulphide/total thiol ratios were significantly higher in the NS patients during relapse and first-attack episode than during remission and in the controls. In addition, the native thiol and total thiol levels, and the native thiol/total thiol ratio were significantly lower, and the disulphide/native thiol and disulphide/total thiol ratios were significantly higher in the NS group during remission than those in the control group. Conclusions: The present findings show that DTDH shifted toward disulphide formation in the NS patients, even during remission. A clearer understanding of the mechanism of this disruption to homeostasis in NS patients might lead to the development of novel therapeutic strategies.
%I Karger Publishers