Y., Long X.-T., Zhao C., Liu Y.-Y., Sun Y.-T., Ma X.-Y., Liu J.-X., Liu Supplementary Material for: A Case-Control Study of the Association of the Polymorphisms of <b><i>MTHFR</i></b> and <b><i>APOE</i></b> with Risk Factors and the Severity of Coronary Artery Disease <b><i>Objectives:</i></b> To explore the association between single-nucleotide polymorphisms (SNPs) in <i>MTHFR</i> and <i>APOE</i> and the risk of CAD and, more importantly, the severity of CAD and the profile of serum lipids, we performed a case-control study in a Chinese Han population. <b><i>Methods:</i></b> A total of 1,207 cases of consecutive CAD-suspected inpatients were recruited, and 406 CAD cases and 231 non-CAD controls were enrolled for the final analysis after screening for exclusion criteria. All subjects had undergone coronary angiography, and the severity of CAD was evaluated by 2 cardiologists according to the Gensini scores. The genotypes of <i>MTHFR</i> and <i>APOE</i>were detected using real-time PCR, and then verified by Sanger sequencing. Environmental risk factors, such as age, sex, smoking, alcohol consumption, hypertension, diabetes, dyslipidemia, and BMI were collected. Statistical analyses (the χ<sup>2</sup> test, binary logistic regression analysis, and ordinal polytomous logistic regression analysis) were performed with SPSS v16.0. <b><i>Results:</i></b> The genotypes of<b><i></i></b>all the subjects included in the CAD and non-CAD groups in this study were successfully detected, with an agreement of 100% with Sanger sequencing. The distributions of genotypes<i> CT</i> and <i>TT</i> at <i>MTHFR C667T</i> were higher in CAD cases than in non-CAD controls (OR 1.99, 95% CI 1.34–2.95; OR 1.77, 95% CI 1.18–2.67; <i>p</i> < 0.05), whereas genotype <i>AC</i> at <i>MTHFR A1298C</i>was lower in CAD cases (OR 0.71, 95% CI 0.50–1.02; <i>p</i> < 0.05). A significant association was observed in genotypes <i>CT</i> and <i>TT</i> at <i>MTHFR C667T</i> and the risk of CAD (OR 1.44, 95% CI 1.27–3.67; OR 1.56, 95% CI 0.88–2.78;<i> p</i> < 0.05). Both genotypes and alleles of <i>APOE</i> were comparable in the CAD cases and non-CAD controls (<i>p</i> > 0.05). The genotype <i>TT</i> at <i>MTHFR C667T</i> and ε4<i>+</i> at <i>APOE</i> were more likely to be found in the CAD subgroup with a Gensini score ≥72 (<i>p</i> = 0.040 and <i>p</i> = 0.028, respectively). Meanwhile, in the patients with genotype <i>TT</i>,<i></i>a higher level of serum Hcy was detected, while genotype ε4+ patients possessed higher levels of serum apolipoprotein E (ApoE) and low-density lipoprotein cholesterol (LDL-C) than other genotypes. <b><i>Conclusion:</i></b> This study revealed that the SNP site of <i>MTHFR C667T</i>is associated<i></i>with the risk of CAD in this Chinese Han population. In addition, the genotypes of <i>TT</i> in <i>MTHFR C667T</i> and ε4<i>+</i>in <i>APOE</i> may increase the severity of CAD, and higher Hcy, LDL-C, and ApoE levels may be involved in this pathogenic process. Coronary artery disease;MTHFR;APOE;Polymorphisms;Risk factor;Severity 2019-06-04
    https://karger.figshare.com/articles/dataset/Supplementary_Material_for_A_Case-Control_Study_of_the_Association_of_the_Polymorphisms_of_b_i_MTHFR_i_b_and_b_i_APOE_i_b_with_Risk_Factors_and_the_Severity_of_Coronary_Artery_Disease/8224130
10.6084/m9.figshare.8224130.v1