Supplementary Material for: A New Physiologic Mouse Model of One Anastomosis Gastric Bypass
2018-11-12T09:17:46Z (GMT) by
<b><i>Background:</i></b> One anastomosis gastric bypass (OAGB) is a modern metabolic operation that has been demonstrated to be a rapid, safe, and effective procedure. As for other bariatric operations, the mechanisms and long-term effects of this procedure remain largely unknown and are difficult to address in human studies. Here, we present a new physiologic mouse model for mechanistic and long-term investigations. <b><i>Methods:</i></b> Six-week-old C57Bl/6 mice were fed a high-fat diet for 12 weeks and scheduled for OAGB or sham operation. Mice were observed for 2 weeks after the operation, and weight and metabolic condition were monitored. <b><i>Results:</i></b> Six mice were used to adapt the surgical technique. Afterwards, another 7 mice were scheduled for OAGB without further complications. The newly established OAGB procedure resulted in significant weight loss and improvement of glucose metabolism 2 weeks after the operation. <b><i>Conclusions:</i></b> The operation presented here is an easy-to-learn and physiologic mouse model of OAGB that can be used for further studies in mice.