000351476_sm_Figure_6.tif.org (39.46 kB)View fileThis item contains files with download restrictions
IMAGE
000351476_sm_Figure_6.gif (31.35 kB)View fileThis item contains files with download restrictions
.ORG
000351476_sm_Figure_5.tif.org (429.95 kB)View fileThis item contains files with download restrictions
IMAGE
000351476_sm_Figure_5.gif (246.05 kB)View fileThis item contains files with download restrictions
.ORG
000351476_sm_Figure_4.tif.org (74.55 kB)View fileThis item contains files with download restrictions
IMAGE
000351476_sm_Figure_4.gif (66.31 kB)View fileThis item contains files with download restrictions
.ORG
000351476_sm_Figure_3.tif.org (234.17 kB)View fileThis item contains files with download restrictions
IMAGE
000351476_sm_Figure_3.gif (153.98 kB)View fileThis item contains files with download restrictions
.ORG
000351476_sm_Figure_2.tif.org (764.06 kB)View fileThis item contains files with download restrictions
IMAGE
000351476_sm_Figure_2.gif (117.33 kB)View fileThis item contains files with download restrictions
.ORG
000351476_sm_Figure_1.tif.org (446.21 kB)View fileThis item contains files with download restrictions
IMAGE
000351476_sm_Figure_1.gif (352.07 kB)View fileThis item contains files with download restrictions
DOCUMENT
000351476_sm_Legends.doc (43.5 kB)View fileThis item contains files with download restrictions
Next page
Previous page
1/1
Switch ViewSwitch between different file views
Thumbnail viewList viewFile view
14 filesFullscreen
Supplementary Material for: Flagellins of Salmonella Typhi and Nonpathogenic Escherichia coli Are Differentially Recognized through the NLRC4 Pathway in Macrophages
posted on 2013-06-27, 00:00authored byYang J., Zhang E., Liu F., Zhang Y., Zhong M., Li Y., Zhou D., Chen Y., Cao Y., Xiao Y.
Flagellin is recognized by both Toll-like receptor (TLR)5 and NAIP5/NLRC4 inflammasome receptors. We hypothesized that the flagellins derived from different bacteria might differentially activate TLR5 and/or NAIP5/NLRC4 signal pathways. To test this, the immune recognition of recombinant flagellins derived from pathogenic Salmonella Typhi (SF) and the nonpathogenic Escherichia coli K12 strain MG1655 (KF) were examined by the activation of TLR5 and NLRC4 pathways in various cell types. While flagellins SF and KF were not distinguishable in activating the TLR5 pathway, KF induced significantly less interleukin-1β production and pyroptotic cell death in peritoneal macrophages than SF, and showed markedly lower efficiency in activating caspase-1 through the NLRC4 pathway than SF. Macrophages may differentially recognize flagellins by intracellular sensors and thereby initiate the immune response to invading pathogenic bacteria. Our findings suggest an active role of flagellin as an important determinant in host differential immune recognition and for the control of bacteria infection.