Supplementary Material for: Functions of Lipopeptides Bacillomycin D and Fengycin in Antagonism of <i>Bacillus amyloliquefaciens</i> C06 towards <i>Monilinia fructicola</i>

In previous studies, <i>Bacillus amyloliquefaciens</i> C06 has been proven to be effective in controlling brown rot of stone fruit caused by <i>Monilinia fructicola.</i> When tested in vitro, cell-free filtrate of <i>B. amyloliquefaciens</i> C06 significantly inhibited mycelial growth and conidial germination of the fungal pathogen. This study aimed to determine the role of the antifungal compound(s) in the cell-free filtrate of <i>B. amyloliquefaciens</i> C06 by an approach combining a DNA-based suppression subtractive hybridization (SSH) method with MALDI-TOF-MS analysis. It was demonstrated that <i>B. amyloliquefaciens</i> C06 harbored two genes, <i>bmyC</i> and <i>fenD</i>, involved in biosynthesis of bacillomycin D and fengycin, two lipopeptides belonging to the iturin and fengycin family, respectively. To determine the roles of bacillomycin D and fengycin of <i>B. amyloliquefaciens</i> C06 in suppressing <i>M. fructicola</i>, the mutants of <i>B. amyloliquefaciens</i> C06 deficient in producing bacillomy- cin D, fengycin or both were constructed, and evaluated in vitro together with the wild-type <i>B. amyloliquefaciens</i> C06. The results indicated that bacillomycin D and fengycin jointly contributed to the inhibition of conidial germination of <i>M. fructicola, </i>and fengycin played a major role in suppressing mycelial growth of the fungal pathogen.