Supplementary Material for: MicroRNA Dysregulation Associated with Red Blood Cell Storage

Introduction: Stored red blood cells (RBCs) undergo
storage lesions involving morphological, physiological
and biochemical changes. MicroRNAs (miRNAs) have
important functions in cell apoptosis and life processes.
Therefore, the aim of this study was to explore potential
roles of miRNAs in the damage of stored RBCs. Methods:
Blood samples were collected from 13 healthy male
O-type donors, and leuko-reduced RBCs were divided
into fresh RBC group and 20-day storage RBC group.
Results: Eight predicted miRNAs with modified expressions
with an intersection ≥ 3 were found dysregulated
in the 20-day storage RBC group and involved in apoptosis
and senescence signaling pathway: miR-31-5p,
miR-196a-5p, miR-203a, miR-654-3p and miR-769-3p
were increased, while miR-96-5P, miR-150-5P and miR-
197-3p were decreased. Evidence associating miR-31-5p,
miR-203a, miR-654 and miR-769 to RBCs or blood in
general
are not available. Conclusions: Dysregulated
miRNAs might represent potential biomarkers to identify
storage lesions, and their detection might help to evaluate
the quality of stored RBCs.