Supplementary Material for: Neuregulin 3 Knockout Mice Exhibit Behaviors Consistent with Psychotic Disorders

Neuregulin 3 <i>(NRG3) </i>is a paralog of <i>NRG1</i>. Genetic studies in schizophrenia demonstrate that risk variants in NRG3 are associated with cognitive and psychotic symptom severity, and several intronic single nucleotide polymorphisms in <i>NRG3</i> are associated with delusions in patients with schizophrenia. In order to gain insights into the biological function of the gene, we generated a novel <i>Nrg3</i> knockout (KO) mouse model and tested for neurobehavioral phenotypes relevant to psychotic disorders. KO mice displayed novelty-induced hyperactivity, impaired prepulse inhibition of the acoustic startle response, and deficient fear conditioning. No gross cytoarchitectonic or layer abnormalities were noted in the brain of KO mice. Our findings suggest that deletion of the <i>Nrg3</i> gene leads to alterations consistent with aspects of schizophrenia. We propose that KO mice will provide a valuable animal model to determine the role of the <i>NRG3</i> in the molecular pathogenesis of schizophrenia and other psychotic disorders.