Supplementary Material for: Sequence and Expression of Two <b><i>cry8</i></b> Genes from <b><i>Bacillus thuringiensis</i></b> INTA Fr7-4, a Native Strain from Argentina

We found and characterized two <i>cry8</i> genes from the <i>Bacillus thuringiensis</i> strain INTA Fr7-4 isolated in Argentina. These genes, <i>cry8Kb3</i> and <i>cry8Pa3</i>, are located in a tandem array within a 13,200-bp DNA segment sequenced from a preparation of total DNA. They encode 1,169- and 1,176-amino-acid proteins, respectively. Both genes were cloned with their promoter sequences and the proteins were expressed separately in an acrystalliferous strain of <i>B. thuringiensis </i>leading to the formation of ovoid crystals in the recombinant strains<i>. </i>The toxicity against larvae of <i>Anthonomus grandis </i>Bh. (Coleoptera: Curculionidae) of a spore-crystal suspension from the recombinant strain containing <i>cry8Pa3</i> was similar to that of the parent strain INTA Fr7-4.



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