appendix.pdf (184.2 kB)
Supplementary Material for: Systems biology of the 2-cell mouse embryo
dataset
posted on 2004-07-14, 00:00 authored by Latham K.E., Radford E.E., Evsikov A.V., de Vries W.N., Peaston A.E., Fancher K.S., Chen F.H., Blake J.A., Bult C.J., Solter D., Knowles B.B.The transcriptome of the 2-cell mouse embryo was analyzed to provide insight into the molecular networks at play during nuclear reprogramming and embryonic genome activation. Analysis of ESTs from a 2-cell cDNA library identified nearly 4,000 genes, over half of which have not been previously studied. Transcripts of mobile elements, especially those of LTR retrotransposons, are abundantly represented in 2-cell embryos, suggesting their possible role in introducing genomic variation, and epigenetic restructuring of the embryonic genome. Analysis of Gene Ontology of the 2-cell-stage expressed genes outlines the major biological processes that guide the oocyte-to-embryo transition. These results provide a foundation for understanding molecular control at the onset of mammalian development.
