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Supplementary Material for: Systems biology of the 2-cell mouse embryo

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posted on 14.07.2004 by Latham K.E., Radford E.E., Evsikov A.V., de Vries W.N., Peaston A.E., Fancher K.S., Chen F.H., Blake J.A., Bult C.J., Solter D., Knowles B.B.
The transcriptome of the 2-cell mouse embryo was analyzed to provide insight into the molecular networks at play during nuclear reprogramming and embryonic genome activation. Analysis of ESTs from a 2-cell cDNA library identified nearly 4,000 genes, over half of which have not been previously studied. Transcripts of mobile elements, especially those of LTR retrotransposons, are abundantly represented in 2-cell embryos, suggesting their possible role in introducing genomic variation, and epigenetic restructuring of the embryonic genome. Analysis of Gene Ontology of the 2-cell-stage expressed genes outlines the major biological processes that guide the oocyte-to-embryo transition. These results provide a foundation for understanding molecular control at the onset of mammalian development.

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