ESR_201805004_0_6_Supplemental_Figure_1.pdf (16.44 kB)
Supplementary Material for: A New Physiologic Mouse Model of One Anastomosis Gastric Bypass
dataset
posted on 2018-11-12, 09:17 authored by Kroh A., Eickhoff R.M., Heise D., Alizai P.H., Rheinwalt K.P., Neumann U.P., Ulmer F.T.Background: One anastomosis gastric bypass (OAGB) is a modern metabolic operation that has been demonstrated to be a rapid, safe, and effective procedure. As for other bariatric operations, the mechanisms and long-term effects of this procedure remain largely unknown and are difficult to address in human studies. Here, we present a new physiologic mouse model for mechanistic and long-term investigations. Methods: Six-week-old C57Bl/6 mice were fed a high-fat diet for 12 weeks and scheduled for OAGB or sham operation. Mice were observed for 2 weeks after the operation, and weight and metabolic condition were monitored. Results: Six mice were used to adapt the surgical technique. Afterwards, another 7 mice were scheduled for OAGB without further complications. The newly established OAGB procedure resulted in significant weight loss and improvement of glucose metabolism 2 weeks after the operation. Conclusions: The operation presented here is an easy-to-learn and physiologic mouse model of OAGB that can be used for further studies in mice.