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Supplementary Material for: Long non-coding RNA brain cytoplasmic RNA 1 induces cisplatin-resistance of cervical cancer cells by sponging microRNA-330-5p and upregulating high-mobility group box 3

posted on 15.06.2022, 09:31 authored by Sun D., Cao R., Han L., Yu X., Wang H., Wang X., Chen X.
Objectives: To find out the function of long non-coding RNA (lncRNA) brain cytoplasmic RNA 1 (BCYRN1) in cisplatin (DDP)-resistance of cervical cancer (CC) cells. Design and Materials, Setting, Methods: BCYRN1 expression in CC and DDP-resistant cells was evaluated, with the association of BCYRN1 and prognosis analyzed. Then, DDP-resistant cells with BCYRN1 knockdown were established and the DDP-resistance of these cells was assessed. BCYRN1 subcellular localization was detected and confirmed. Besides, binding relation of BCYRN1 and microRNA (miR)- 330-5p and between miR-330-5p and high-mobility group box 3 (HMGB3) were examined and verified. Moreover, role of miR-330-5p and HMGB3 in the mechanism of BCYRN1 modulating DDP-resistance of CC cells was detected. In addition, xenograft transplantation was conducted to confirm the effect of BCYRN1 in CC cell DDP-resistance. Results: BCYRN1 was overexpressed in CC, which resulted in poor prognosis and DDP-resistance. BCYRN1 knockdown in DDP-resistant cells downregulated DDP-resistance. Mechanically, BCYRN1 sponged miR-330-5p to strengthen HMGB3 mRNA level. Besides, miR-330-5p underexpression or HMGB3 overexpression reversed the function of BCYRN1 knockdown in inhibiting DDP-resistance of CC cells. Eventually, BCYRN1 knockdown reduced DDP-resistance of CC cells in vivo. Limitations: There are still some deficiencies in the research; for example, whether there are other miRs working as the downstream genes of BCYRN1 in the ceRNA interaction is not fully clarified; nor the other downstream mechanism of miR-330-5p. Besides, the experimental findings and application into practice need extensive validation. Conclusions: BCYRN1 knockdown could disrupt DDP-resistance of CC cells through upregulating miR-330-5p to suppress HMGB3 mRNA level.