Supplementary Material for: Predictive value of optical coherence tomography biomarkers on visual outcomes in patients with retinal vein occlusion

Purpose: To evaluate the prognostic significance of different optical coherence tomography (OCT)-imaging biomarkers in predicting visual outcomes in patients with retinal vein occlusion (RVO). Methods: This was a retrospective study conducted on consecutive patients diagnosed with RVO, who were referred to or treated at a Third-level university hospital. The primary endpoints were the mean change in best-corrected visual acuity (BCVA) from baseline to the month-6, month-12, and month-18 follow-up visits, and the influence of OCT-imaging biomarkers, including ellipsoid zone (EZ) alterations, disorganization of the retinal-inner-layers (DRIL), hyperreflective foci (HRF), neurosensorial-detachment (NSD), and fibrosis on functional outcomes, defined as a BCVA improvement of ≥5 ETDRS letters. Results: One-hundred-and sixty-three eyes were included. In the overall-study sample, mean (Standard-error) BCVA increased significantly from 49.6(1.8) letters at diagnosis to 54.6(1.8) letters at month-6 (p=0.0069), 54.3(1.9) letters at month-12 (p=0.0071), and 53.6(1.9) letters at month18 (p=0.0313). In multivariate analysis, the presence of EZ alterations at diagnosis (OR: 0.19; p = 0.0008) and their persistence to month-12 (OR: 0.34; p=0.0043) were associated with a reduced probability of achieving a BCVA improvement of ≥5 ETDRS letters at month-12. By month 18, significant predictors of visual outcomes included EZ alterations at diagnosis (OR: 0.19; p=0.0008), sustained EZ alterations through 18 months (OR: 0.34; p=0.0043), and DRIL at month-18 (OR: 0.38; p = 0.0321). Conclusions: The presence of EZ alterations at diagnosis and their persistence at 12 and 18 months, along with DRIL at 18 months, were adverse prognostic markers for BCVA outcomes in patients with RVO.